Fig. 4
From: AIMTOR, a BRET biosensor for live imaging, reveals subcellular mTOR signaling and dysfunctions
Targeting AIMTOR to various subcellular localizations. a–d Fluorescence microscopy of living differentiated C2C12 myotubes expressing either cytosolic (NES) (a), nuclear (NLS) (b), mitochondrial (MITO) (c), or lysosomal (LYSO) AIMTOR (d). The left and middle channels show in a gray scale LUT YPET fluorescence of AIMTOR and either mitochondria (a–c) (labeled with mitotracker) or lysosomes (labeled with lysotracker Red99) (d), respectively. For the merge image on the right, the YPET fluorescence of AIMTOR is in green and mitochondria or lysosomes are in magenta. Scale bar represents 10 μm