Fig. 3
GABAA receptor blockade with bicuculline (BIC) differentially induces network burst events in striatal cultures of WT or HD. a Spontaneous neuronal activity was recorded during 10 min in basal conditions and after addition of bicuculline (BIC, 60 μM). The plots show representative average fluorescence trace, and reconstructed spikes from neurons are represented in raster plots for WT (top) and HD (bottom) for baseline (left) and after system wide disinhibition with BIC 60 (right). Vertical blue bars highlight network bursts. b Percentage of active neurons before and after the addition of BIC. c Percentage of active neurons participating in network bursts before and after the addition of BIC and d average inter-burst interval (IBI). e Global activity rate of the cultures. f Fraction of independent spikes. Each circle represents a single experiment (b, e, f: STR WT n = 11; STR HD n = 10; CTX WT n = 19; CTX HD n = 22) (c, d: STR WT n = 4; STR HD n = 5; CTX WT n = 19; CTX HD n = 22), thick line the mean, thick shaded area the standard error of the mean, and thin shaded area the standard deviation. Statistical analysis was performed using mixed ANOVA and posterior post hoc test with Bonferroni’s multiple comparison correction. Only * for post hoc test are shown, *p < 0.05, **p < 0.01, ***p < 0.001