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Fig. 2. | BMC Biology

Fig. 2.

From: Real-time tracking of stem cell viability, proliferation, and differentiation with autonomous bioluminescence imaging

Fig. 2.

Stem-luxCDEF/Stem-luxAB-induced autobioluminescence faithfully recapitulates the results of common assays without necessitating sample destruction. a The fold change in autobioluminescence relative to the background correlates strongly with the initial cell seeding density (R2 = 0.93) to allow a continuous population size determination. N = 6 replicates. b The fold change in autobioluminescence relative to the background correlates strongly with the fold change in MTT absorbance (570 nm) relative to the background (R2 = 0.98). N = 6 replicates. c iPSCs challenged with the indicated doses of doxorubicin report viability without perturbation similarly to the destructive MTT assay. d Viability measurements from the two test formats show a strong correlation between the results. The inset shows the correlation between test results under low viability conditions as indicated in the boxed section of the main plot. Values are representative of N = 3 replicates. Error bars represent the standard error of the means. p/s/cm2/sr; photons/s/cm2/steradian. Data is available at https://osf.io/h5qzj/ [15]

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