Fig. 5

Histopathology following infection with dengue virus (DENV) in mice with varying nutritional status. Six-week-old female C57BL/6 J mice were fed for 8–10 weeks on a control (healthy weight), high-fat (obese), or low-protein (protein-energy malnutrition, PEM) diet and then treated with 1 mg of antibody to block interferon receptor signaling (IFNAR blocking antibody). The next day, mice were infected with DENV2 Puo-218. a Histology scores from the spleen and liver collected 2 days post-infection. Values are presented as medians with the minimum and maximum of the range from groups of four animals. Statistical comparisons were made using the Kruskal-Wallis test with Dunn’s multiple comparisons test. *p < 0.05 and **p < 0.01. b Representative images of the liver and spleen collected before and after infection with DENV2. Black-filled arrows represent areas of inflammation. Black and white arrows represent areas of lipidosis. Scale bar, 100 μM. Data presented are from one biological replicate