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Fig. 6 | BMC Biology

Fig. 6

From: Stability of Begomoviral pathogenicity determinant βC1 is modulated by mutually antagonistic SUMOylation and SIM interactions

Fig. 6

SUMO/SIM mutants of βC1 block viral systemic movement. a Southern blot viral replication assay in local leaves of transgenic plants expressing p35S::GFP-βC1 or its SUMOylation motif mutants. Infectious DNA A clones were used for infection. Total genomic DNA was treated with S1 nuclease before blotting. b Viral replication assay using SIM mutants of GFP-βC1. c Southern blot showing viral replication and systemic movement with DNA β and SIM/SUMO mutated βC1 variants incorporated in DNA β in local and systemic leaves of N. benthamiana. d Phenotypes of SyYVCV + DNA β or SIM/SUMO mutated βC1 incorporated in DNA β in N. benthamiana (top) and N. tabacum (bottom). Images were taken at 39 DPI. Size bar 5 cm

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BMC Biology

ISSN: 1741-7007

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