Fig. 6.

Hierarchical clustering of the different sample groups, treated with chloroquine (CQ) (blue), DHA (green), azithromycin (Az) (light blue), GSK-5 (purple), GSK-71 (yellow), GSK-66 (grey) and ethanol control (red). Vertical clustering displays similarities between sample groups, while horizontal clusters reveal the relative abundances of the 50 most significantly different metabolites from experiment 1. The significantly differentially regulated metabolites are further classified into three different groups, the CQ-like peptides (blue line), TCA cycle (red line) and haemoglobin-derived peptides (orange lines). All compounds were tested with three technical replicates. White indicates no change, while red and blue indicates increased and decreased abundances respectively. Ward’s minimum variance method algorithm was used to generate the hierarchical cluster analysis