Fig. 5

AAD is negligible in injured cortical axons in vivo. a No evidence of AAD within 60 min of injury when the length of severed cortical axon distal segments is monitored with a membrane marker (Thy1-L15). Note length stability in all but one axon (red line), indicating absence of fragmentation over the first 60 min post-lesion (n = 16 axons, 7 mice). b Example of the retraction of the severed axon away from the lesion site when observed with a cytosolic GFP marker (GFP-M line). Note the absence of fragmentation over the first 60 min post-lesion. Arrowheads indicate intact axon. Axon ending is on the right edge of the frame. Scale bar = 10 μm. c Quantification of the distance from the lesion site to the distal axon stump in cytosolic GFP labelled axons (Thy1-GFPM line) at the indicated post-lesion times (n = 10 axons, 7 mice)