Fig. 7

Ingenuity Pathways Analysis network summary predictions. a Ingenuity Pathways Analysis was used to predict further genes associated with glucose homeostasis based upon the 22 genes found to be differentially expressed in the microarray. b Predicated genes were analysed by RT-qPCR in WT and Phospho1^−/− primary calvarial osteoblast’s. Results were normalised to the Atp5b housekeeping gene. Data are represented as mean ± S.E.M (n = 3 replicates). *P < 0.05, **P < 0.01, ***P < 0.001. Red = Upregulated. Green = Downregulated (the darker the shade of green and red colour indicates a more extreme up/down regulation, conversely the paler the shade indicates a more subtle up/down regulation. Dashed line = indirect interaction (blue = inhibition, yellow = findings underlying the relationship are inconsistent with the state of the downstream node, grey = Ingenuity Pathways Analysis prediction) and the data were assessed in (b) using a two-tailed unpaired t test