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Fig. 4 | BMC Biology

Fig. 4

From: Hyperactivated Wnt-β-catenin signaling in the absence of sFRP1 and sFRP5 disrupts trophoblast differentiation through repression of Ascl2

Fig. 4

Trophoblast-specific stabilization of β-catenin impairs EPC trophoblast differentiation with excessive TGC differentiation. a Average litter sizes of WT and Cyp19-cre female mated with Ctnnb1f(EX3)/+ male mice, respectively. *P < 0.05. b Genotyping of the newborns from the intercrosses of Cyp19-cre female and Ctnnb1f(EX3)/+ male mice. c, d Average embryo degeneration rate and representative uteri of WT and Cyp19-cre female mated with Ctnnb1f(EX3)/+ male mice, respectively, at E11.5. Arrowheads indicated degenerated embryos. *P < 0.05. e Genotyping of the remaining survived embryos from the intercrosses of Cyp19-cre female and Ctnnb1f(EX3)/+ male mice at E11.5. f HE and CK staining of Cyp19-cre and Cyp19-cre; Ctnnb1f(EX3)/+ placental sections at E9.5. gi The expression of Ascl2, Hand1, Tpbpα and Pl1 in the Cyp19-cre and Cyp19-cre; Ctnnb1f(EX3)/+ placentas at E9.5, were detected by in situ hybridization and qRT-PCR, respectively. Values are normalized by Gapdh expression level and indicated as means ± SD. N = 3. *P < 0.05. In ac and d, numbers within and above the bars indicated the number of pregnant mice and embryos examined, respectively. Images in fh are representatives of at least three independent experiments. Dec, decidua; Cp, chorionic plate; Sp, spongiotrophoblast; TGC, trophoblast giant cell. Scale bar, 100 μm

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