Fig. 5
From: Bioengineered human skeletal muscle capable of functional regeneration
Dynamics of cell populations following injury. a Representative images showing staining left to right, Nuclei (DAPI, blue), MyoD (green) and overlay image. Scale bar represents 25 μm. Graph displays percentage of MyoD+ nuclei across recovery. b Representative images showing staining left to right, Nuclei (DAPI, blue), Pax7 (green) and overlay image. Scale bar represents 25 μm. Graph displays percentage of Pax7+ nuclei across recovery. c Normalised nuclei per mm2 across recovery, mean value at control – 1471 mm− 2. d RT-PCR data displayed as ΔΔCT values for myogenic genes Pax7 and MyoG across recovery. a–e All graphs display means ± S. D, individual repeat means are displayed as points. Dashed line represents level at control on all graphs. Statistical significance from control is denoted as *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001, n = 15 samples across 5 repeats