Fig. 3
From: The CBP KIX domain regulates long-term memory and circadian activity
Differential gene expression analysis in the dorsal hippocampus of wildtype mice following learning in fear conditioning and water maze compared to homecage controls. a Experimental scheme: RNA-Seq analysis performed after learning in Fig. 2 was compared to RNA extracted from WT homecage animals. To identify the genes altered by learning, we compared WT contextual fear conditioning (n = 4) with WT homecage (n = 4) and WT MWM (n = 3) with WT homecage (n = 3). b Volcano plot illustrating differentially expressed genes between WT learning and WT homecage mice. c KEGG pathway enrichment network analysis showing significant KEGG pathways enriched in the upregulated genes. Top significant pathways are shown in the bar graph. MAPK signaling pathway (p = 0.0019). d Heat map of IPA upstream regulator analysis on differentially expressed genes in CBPKIX/KIX mice following training. Most significant regulators are on the top of the heat map. CREM is the top-predicted upstream regulator of differentially expressed genes (FDR < 0.05) in the WT learning group compared to homecage controls (predicted activation, z-score = 2.854, p = 2.54 × 10−21). CREB1 also appears as an upstream regulator (predicted activation, z-score = 3.715, p = 3.97 × 10−13). e, f Known interactions of differentially expressed genes (FDR < 0.05) in learning group vs homecage predicting CREB (e) and CREM (f) as upstream regulators