Fig. 2
From: CloneSifter: enrichment of rare clones from heterogeneous cell populations
Tracking clonal dynamics in D458 cells using a 26 nt sgRNA-barcode library. a Relative barcode abundance in D458 cells before treatment (early time point, ETP) and after treatment with 2 μM JQ1 (5 replicates) or DMSO vehicle (5 replicates). b, c The sgRNA-barcode library is able to track a heritable phenotype. b Comparison of barcode abundance across conditions for barcodes enriched in JQ1, DMSO, or JQ1 and DMSO replicates. Barcode enrichment was defined based on the median rank across replicates (Methods). c The majority of JQ1-enriched barcodes were detected across all replicates at an abundance > 10−5. The raw barcode read counts are provided as a CSV file in Additional file 5: Table S7-barcode_counts.csv and the raw histograms for barcode counts are available in Supporting Data 2: barcode histograms [15]