Fig. 3

ATP stimulates P2X7R-Paxillin interaction. a HEK293T cells were co-transfected with pFlag-P2X7R and pHA-Paxillin or pFlag-Pannexin-1 and pHA-Paxillin. b TPA-differentiated THP-1 macrophages were mock-treated or treated with ATP (5 mM) for 30 and 60 min. c HEK293T cells were co-transfected with pFlag-P2X7R and pHA-Paxillin and treated with DMSO or ATP (5 mM) for 2 h. d HEK293T cells were co-transfected with pFlag-P2X7R and pHA-Vector, pHA-Paxillin, pHA-Paxillin(Y31A), and pHA-Paxillin(Y118A). e HEK293T cells were co-transfected with pFlag-P2X7R and pHA-paxillin(Y31A) and treated with DMSO or ATP (5 mM) for 2 h. f HEK293T cells were co-transfected with pFlag-P2X7R and pHA-Paxillin(Y118A) and treated with DMSO or ATP (5 mM) for 2 h. g The diagrams of P2X7R, P2X7R(26–595), P2X7R(47–595), P2X7R(335–595), and P2X7R(356–595) (left). h HEK293T cells were co-transfected with pHA-Paxillin and pFlag-P2X7R, pFlag-P2X7R(26–595), pFlag-P2X7R(47–595), pFlag-P2X7R(335–595), and pFlag-P2X7R(356–595). i The diagrams of P2X7R(26/27/29/30/31/32/33/34/35/36–595). j HEK293T cells were co-transfected with 2 μg pHA-Paxillin and 6 μg pFlag-P2X7R(26–595), pFlag-P2X7R(27–595), pFlag-P2X7R(29–595), pFlag-P2X7R(30–595), pFlag-P2X7R(31–595), pFlag-P2X7R(30–595), pFlag-P2X7R(32–595), pFlag-P2X7R(33–595), pFlag-P2X7R(34–595), pFlag-P2X7R(35–595), and pFlag-P2X7R(36–595). k HEK293T cells were co-transfected with pFlag-P2X7R and pHA-Paxillin or pFlag-P2X7R(K30A) and pHA-Paxillin