Fig. 1

The overall structure of the ScADAT2/3 heterodimer and inter-subunit interactions. a The ScADAT2/3 heterodimeric structure shown in the ribbon rendering in two orthogonal views. The N-lobe and PCD of ScADAT3 are colored light orange and yellow, respectively, and circled; RLD is shown in magenta. The catalytic domain (CD) and the C-terminal protrusion of ScADAT2 are colored cyan and red, respectively. The N- and C-termini are indicated. b Superimposition of the AaTadA homodimer structure (PDB 1WWR, gray) with that of the ADAT2/3 heterodimer. Their major differences are the additions as the C-terminal tail of ScADAT2 and the N-lobe of ADAT3, which are colored red and magenta, respectively. The zinc ions were shown as the red spheres. c The interfacial hydrogen bonding network between the heterodimers as indicated by the red dashed lines (cutoff distance 3.5 Å). d, e The hydrophobic interactions as indicated by the dashed magenta lines (cutoff distance 4.7 Å)