Fig. 5
From: Lethal (2) giant discs (Lgd)/CC2D1 is required for the full activity of the ESCRT machinery
Comparison of shrub clones in the absence or presence of one copy of BACshrub-CHMP4B in the genome with lgdd7 mutant cell clones. Clones are labelled by the absence of GFP. (A–E) shrub mutant cell clones are rare and comprise only a few cells. (A, B) Overview of the notal region of a wing disc bearing a shrub mutant clone. (C–E) Magnification of the region boxed in (A, B). A small shrub mutant clone is boxed and shown at higher magnification in the insert. The few surviving mutant cells contain enlarged Notch-positive MEs (E). In contrast, large orphan wild-type twin-clones (GFP-positive (arrow in A, B and outlined in yellow D, E) are present in these discs. (F–H) shrub mutant cell clones partially rescued with one copy of BACshrub-CHMP4B. (F) Several large mutant clones can be observed, indicating that the lethality is suppressed. (G, H) Magnification of the region boxed in (F) which includes a large clone. The partially rescued cells of the clone display a similar endosomal defect as the cells of lgd mutant clones, shown in (I–K). In both cases, the enlarged MEs of the mutant cells accumulate Notch. (K, K’) Note that the levels of Shrub in the lgd mutant cells are similar to that of the neighbouring wild-type cells. Scale bars (A, B, F) 50 μm, (C–E, G–K) 10 μm. At least ten wing imaginal discs were analysed for each genotype