Fig. 2
From: Feedback inhibition of AMT1 NH4+-transporters mediated by CIPK15 kinase
CIPK15 inhibited AmTryoshka1;3 LS-F138I activity in yeast. a Schematic representation of AmTryoshka1;3 LS-F138I [15]. b CIPK15 reduced NH4+-triggered AmTryoshka1;3 LS-F138I [15] responses in yeast. Amtryoshka1;3 LS-F138I was co-expressed with control (vector only), CIPK15, and CIPK15m (inactive mutant). Results of normalized fluorescence ratio (normalized to buffer control = 1, λexc 440 nm, ratio = FI510nm/570nm) after addition of NH4Cl as represented by box and whiskers (mean ± SE, n = 8). Center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by Prism software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, outliers are represented by dots. p, significant change as shown in the figure (two-way ANOVA followed by Tukey’s post-test). PM, plasma membrane