Fig. 1

Thalidomide elicits mechanical and cold, but not heat, hypersensitivity that is dependent on TRPA1 and TRPV4. a–c Dose- and time-dependent mechanical and cold allodynia and heat hypersensitivity following intraperitoneal (i.p.) injection of thalidomide (Thal, 1, 10, 50, and 100 mg/kg) or Veh. d–f Mechanical allodynia at day 7 following Thal (50 mg/kg, i.p.) and Veh and after the administration of HC-030031 (HC03, 100 mg/kg, i.p.), HC-067047 (HC06, 10 mg/kg, i.p.), a combination of HC03 (100 mg/kg, i.p.) and HC06 (10 mg/kg, i.p.), or Veh. g, h Cold allodynia at day 7 following Thal (50 mg/kg, i.p.) or Veh and after the administration of HC03 (100 mg/kg, i.p.), HC-067047 (HC06, 10 mg/kg, i.p.), or Veh. BL, baseline. Data are mean ± SEM, n = 6 mice. *P < 0.05 vs. Veh Thal or Veh Thal/Veh HC03/Veh HC06; ^§P < 0.05 vs. Thal/Veh HC03/Veh HC06. Two-way ANOVA followed by Bonferroni’s post hoc test