Fig. 2

Genome-wide analysis of temperature-dependent transcriptome responses to photoperiod in quail. Experimental design showing the 3 time-points each sampled after 4 weeks of the target photoperiod (circled) with RNA-Seq at n = 4 a. Intersection of DEGs between LD 23 °C vs SD 23 °C and LD 23 °C vs SD 9 °C b. Volcano plots comparing LD 23 °C vs SD 23 °C showing 71 up (yellow) and 42 down (blue) DEGs c and LD 23 °C vs SD 23 °C d. Grey labels do not pass fold change threshold at 23 °C. Temperature-dependent effects on fold change in DEGs when comparing SD at 23 °C and SD 9 °C. Arrows point from 23 to 9 °C and indicate a significant amplifying (green) or dampening (orange) effect of 9 °C on photoperiod response e significantly enriched pathways in DEG genes at LD vs SD 23 °C (grey) and LD vs SD 9 °C (teal) q-value thresholds f. Network of up (yellow), down (blue) and no significant change (white) regulated inter-connected genes (LD vs SD) using the String database. The left side of a node indicates the expression change at 23 °C and right at 9 °C. Edges are weighted by the combined score, and green edges represent experimental support g. Summary of upregulated and downregulated pathways h