Fig. 2
Cytosolic redox sensor Grx1-roGFP2 is not detectably disturbed upon thapsigargin-induced Ca2+ release. Fluorescence ratio changes of cytosolic Grx1-roGFP2 transiently expressed in HEK293. Traces correspond to data recorded from one cell; traces were obtained from two independent experiments. Cells were pretreated for 3 h before imaging with 100 μM of the GR inhibitor carmustine (BCNU) to prevent GSSG re-reduction. One micromolar TG were applied to cells as indicated by the arrow. At the end of the experiment, 500 μM diamide (Dia) and 20 mM DTT were added to ensure the functionality of the probe