Fig. 5

Osh3 co-localizes with Hsp104 under heat stress conditions. a Cells expressing Osh3-GFP (green) and Hsp104-mCherry (magenta) grown at 26 °C (upper panel) and after a 10 min 42 °C heat shock (lower panel). Scale bar, 5 μm. b Quantitation of Osh3-GFP and Hsp104-mCherry co-localization at 26 °C and 42 °C heat shock. Graphs show the means and standard deviations from three independent experiments (t test, ****p < 0.0001). The points present values of individual image frames (> 10 cells/frame). Number of frames analyzed: 26 °C n = 36, 42 °C n = 36