Fig. 4

The metabolic switch is achieved through the regulation of enzymes that work on branch points between competing pathways. a Absolute quantification of 43 enzymes of the TCA and glyoxylate cycle, mitochondrial pyruvate metabolism, gluconeogenesis, and glycolysis in daf-2 dauers grown at 25 °C compared to daf-2 L3 larvae at 15 °C. Means ± standard deviation of 3 biological replicates with 2 technical replicates each. b Schematic representation of the pathway that converts lipids to carbohydrates with absolute quantification of the enzymes that operate at the branch points in daf-2 L3 larvae at 15 °C and animals of daf-2 and daf-2;aak-2 grown at 25 °C with or without DA. Red arrows and green circles represent the competing reactions at the point of divergence between (1) TCA and glyoxylate pathway and (2) the recycling of oxaloacetate into the TCA/glyoxylate cycle or its entry into gluconeogenesis. Means ± standard deviation of 3 biological replicates with 2 technical replicates each. Additional file 9: Tab. S1 contains the molar abundances of all 43 proteins in biological replicates for all tested conditions