Fig. 6

Dendritic spine characteristics of CRH neurons. A The dendritic spine characteristics of aspiny CRH neurons in brain regions of the cortex, VMPO, and SCN. The thin spines are indicated by the arrows and the filopodia-like spines are indicated by the yellow arrowheads. B The dendritic spine characteristics of spiny CRH neurons in brain regions of the LSD, hippocampus, BST, and CeA. The thin spines are indicated by the arrows and the mushroom-like spines are indicated by the white arrowheads. The inserted images are magnified images from the dotted boxes (scale bar: 50 μm and 5 μm for the inserts). C The difference in dendritic spine density between the BST (n = 11) and CeA (n = 11) (P < 0.0001, t = 5.467). D (a) The GAD67-GFP-positive CRH neurons in the BST are spiny. a is a magnified image from the dotted box in D. E The aspiny CRH neurons in the BST. F (b) and (c) The GAD67-GFP-positive (b) and -negative (c) CRH neurons in the CeA are spiny. b and c are magnified images from the dotted box in F. The inserted images are magnified images from the dotted boxes (scale bar: 50 μm for D and F, 10 μm for E and a–c. and 5 μm for the inserts). G–H The retrograde-labeled CRH neurons in the AM (H, d) by injecting fluorogold in the mPFC (G) are aspiny (e, the thin spines are indicated by the arrows). d and e are magnified images (scale bar: 1 mm for G and H, 50 μm for d, and 5 μm for e)