Fig. 1

Lifespan and healthspan regulation by H3K27 methylases and demethylases. a The KDM6 family of H3K27 demethylases comprised utx-1, jmjd-3.1, jmjd-3.2 and jmjd-3.3 in C. elegans (TPR, tetratricopeptide repeat; JmjC, Jumonji C domain). Animals containing mutant alleles of mes-2, jmjd-3.2 or utx-1 were analysed for both lifespan (b, c, e, f) and healthspan (d, g). b Both mes-2(tm5007) and mes-2(ok2480) mutants live significantly longer than the N2 control strain (***p = 0.0003 and ****p < 0.0001, respectively). c Worms that are either homozygous or heterozygous for the mes-2(bn11) allele have extended lifespan when compared to either N2 (****p < 0.0001 and ***p = 0.0005, respectively) or the balancer-containing (SP127) control (****p < 0.0001). d The three mes-2 mutants display an increased thrashing rate, indicative of better health, at day 12 and day 15 of adulthood compared to the N2 control strain (p < 0.005 in all cases). e, f: jmjd-3.2(tm3121) mutant worms (e) and utx-1(tm3118) heterozygotes (f) display extended lifespan compared to the N2 control (**p = 0.005 and ****p < 0.0001, respectively). g jmjd-3.2(tm3121) mutants and utx-1(tm3118)/+ heterozygotes display increased thrashing rates, indicating better health, at day 12 and day 15 of adulthood when compared to N2 controls (p < 0.001 in each case) (see Additional file 3: Table S2 for the full statistical analysis of lifespan data, including repeats). For thrashing assays, n ≥ 10 worms, measured at least 3 times for each strain. Box and whisker plot represents the first and third quartile and minima and maxima of the data points