Fig. 5.

Myo1e colocalizes to dorsal membrane ruffles. a Boxplot of the enrichment ratio of investigated proteins in membrane ruffles, as determined by the equation above (see the ‘Methods’ section). Bars show range, the inner box shows S.D., and inner bar shows the mean value. n = 4 cells each, > 40 ruffles. b Normalized PA-Rac1 and Myo1e intensities with time (seconds) for two example membrane ruffles. c Correlation of PA-Rac1 and Myo1e signals. Correlation measured from 35 ruffles. d Top, MIP and cross-section of PA-Rac1 (blue) and Myo1e (yellow). Pink boxes refer to zoomed panels, bottom. Red arrows highlight colocalizing dorsal ruffles. Green box refers to zoomed time series (e). Scale bar = 5 μm. e Time series of PA-Rac1 (blue) and Myo1e (yellow). Scale bar = 1.25 μm