Fig. 5

HSF-1 overexpression partially rescues yolk endocytosis, neuronal signaling, and recovery of offspring following chronic HS. a, b Coomassie-stained SDS-PAGE reveals that exposing adults to 24-h 28 °C HS results in a loss of YP170 in WT (N2) embryos that is rescued in HSF-1 overexpression (HSF-1 O/E; EQ140) embryos. a Representative image from one of three independent trials. Arrows indicate YP170, YP115, and YP88 bands. The rme-4(b1001) mutant YP170::GFP strain was included for YP band identification. MW: molecular weight marker; WT: wild-type. b Quantification of a shows the relative change in each YP level upon HS. Data represent the mean ± SD of n = 3 independent trials. c HSF-1 O/E worms show restored sensitivity to aldicarb compared to WT worms that show resistance to aldicarb following exposure to 48-h 28 °C HS. Neither strain shows significant changes in drug response after 24-h 28 °C HS compared to 20 °C controls. Data represent the mean ± SD of n = 3 independent trials. d–f HSF-1 O/E worms show improved reproductive function compared to WT during a 20 °C recovery period after a 24-h 28 °C HS, as shown by the percent of adults that recovered viable offspring (d) and the average number of eggs laid (e) and viable offspring produced (f) by each worm. Data in d represent the mean ± SD of n = 3 independent trials. Data in e and f represent the mean ± SD of n = 30 worms collected across three trials. * p < 0.05, ** p < 0.01 (Student’s t test)