Fig. 6.

Chaperone titration and disrupted endocytosis link chronic heat stress with organismal phenotypes. Chronic protein-folding stress causes a titration of chaperones and subsequent disruption in endocytosis across cell types, ultimately leading to diverse physiological defects. Here, we provide evidence of this process in endogenous yolk levels and a fluorescent yolk reporter for embryos, a GFP reporter for coelomocytes, and functional assays for neurons coupled with HSF-1-mediated rescue of endogenous yolk uptake and neuronal signaling