Fig. 7.
Lack of roof plate formation in the absence of Notch signaling. In situ hybridization of definitive RP markers on transverse sections through the dorsal neural tube at the level of the forelimb (a, b, e-n, o, p, s-x) or hindbrain (c, d, q, r) in wildtype (WT) and Wnt1-Cre; Mib1fl/fl mouse embryos at E11.5 and E10.5. Expression of all RP in situ hybridization markers are missing from mutants at E11.5 and E10.5: Rspo1 (a, b and o, p); BAMBI (c, d and q, r); GDF7 (e, f and s, t); Raldh2 (g, h and u, v); Slit1 (i, j and w, x); Msx1 (k, l). m, n Combination of adjacent sections of Lmx1a in situ hybridization (blue) and immunostaining for Dll1 (black) showing complementary expression of the two markers at E11.5 in WT embryos but loss of Lmx1a in mutants with a corresponding dorsal expansion of Dll1. Bar = 100 μm