Skip to main content

Table 1 Preparation of Prdm9 mutant SHR animals by mRNA injections

From: Rat PRDM9 shapes recombination landscapes, duration of meiosis, gametogenesis, and age of fertility

Experiment   1 2 Total
mRNA concentration 2 ng/μl 5 ng/μl Both
Injected zygotes 121 113 234
Transferred embryos 2c/1c* 57/18 62/21 119/39
Female recipients 6 7 13
Pregnant recipients 3 3 6
Live progeny born 15 8 23
Mutants 3 1 4
  1. *2-cell stage/1-cell stage transferred into recipients after 20 h of in vitro culture post injection