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Table 1 Preparation of Prdm9 mutant SHR animals by mRNA injections

From: Rat PRDM9 shapes recombination landscapes, duration of meiosis, gametogenesis, and age of fertility

Experiment  

1

2

Total

mRNA concentration

2 ng/μl

5 ng/μl

Both

Injected zygotes

121

113

234

Transferred embryos 2c/1c*

57/18

62/21

119/39

Female recipients

6

7

13

Pregnant recipients

3

3

6

Live progeny born

15

8

23

Mutants

3

1

4

  1. *2-cell stage/1-cell stage transferred into recipients after 20 h of in vitro culture post injection