Fig. 4

Comparison of DI244 production in a STR and a shake flask. A STR was inoculated with MDCK-PB2(sus) cells from a shake flask preculture at a VCC of 2E+6 cells/mL. Cells were infected with purely clonal DI244 seed virus at MODIP 1E−2. a HA titer, b DI244 titer (plaque assay in MDCK-PB2(adh)), c DI244 vRNA level (determined by real-time RT-qPCR), d VCC. For comparison, DI244 production in a shake flask at the same MODIP (Fig. 1) is shown