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Table 2 ATP reporter constructs designed and used in this study

From: Measuring and modeling energy and power consumption in living microbial cells with a synthetic ATP reporter

Name

Plasmid copy number

RBS strength design

LC-F

Low copy

16,000, medium strength

LC-G

Low copy

50,000, high strength

HC-M

High copy

7000, low strength

HC-E

High copy

16,000, medium strength

HC-con

High copy

16,000, medium strength

  1. The RBS strength is indicated as the translation initiation rate (arbitrary unit) designed by the RBS calculator and is arbitrarily defined as low, medium, and high strength in this study. All reporters were made with the rrnB P1 promoter except the control plasmid HC-con that used the strong constitutive promoter T7A1
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BMC Biology

ISSN: 1741-7007

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