Fig. 1

Anti-PD-L1 plus anti-TGF-β leads to tumor regression in mice. a–d Hu-PD-L1 KI mice bearing s.c. Hu-PD-L1-MC38 tumors (n = 6 per group) were dosed I.P. biweekly for 3 weeks with PBS, aPD-L1 (2 mg/kg; atezolizumab), or aPD-L1 plus aTGF-β (10 mg/kg; 1D11). a Average MC38 tumor volume ± SEM is shown. P values were determined using Wilcoxon rank sum test, comparing tumor sizes on day 18. b Spider plots showing MC38 tumor volume for individual mice over time. c Survival plot for the MC38 study. P values were determined using log-rank test. d CD3 IHC score in MC38 tumors by immunohistochemistry (IHC). Each point is the IHC score representing the density of CD3+ cells from an individual mouse. The horizontal black bars of the box plots indicate median score, while the lower and upper hinges correspond to the first and third quartiles, respectively. P values were determined using Wilcoxon rank sum test. e–h Mice bearing orthotopic EMT6 tumors were treated with PBS, aPD-L1 (10 mg/kg for the first dose with each subsequent dose at 5 mg/kg; atezolizumab), aTGF-β (10 mg/kg; 1D11), or aPD-L1 plus aTGF-β. For all treatments, the first dose was administered I.V. on day 0 and the eight subsequent doses were administered I.P. three times per week. n = 12 mice per group, including 3 mice per group taken down early on day 8 for scRNA-seq analysis. e Average EMT6 tumor volume ± SEM is shown. P values were determined using Wilcoxon rank sum test. Mice removed early for scRNA-seq analysis were not included in the average. f Spider plots showing EMT6 tumor volume for individual mice over time. Mice sacrificed for scRNA-seq (n = 3 per group) are shown in red. g Survival plot for the EMT6 study. P values were determined using log-rank test. h CD3 IHC score in EMT6 tumors. Each point is the IHC score representing the density of CD3+ cells from an individual mouse. The horizontal black bars of the box plots indicate median score, while the lower and upper hinges correspond to the first and third quartiles, respectively. P values were determined using Wilcoxon rank sum test. Non-significant p values are not shown for all panels