Fig. 4

Concatenated mitochondrial phylogenetic analysis confirms EU17/18 as a member of an early-diverging euglenozoan lineage. Mitochondrial-encoded protein sequences were predicted from EU17/18 and EU2 mitochondrial contigs and subjected to phylogenetic analysis. The alignment, based on a previously published study including 15 mitochondrion-encoded proteins [33], contained 37 taxa and 4325 amino acid positions. EU17/18 and EU2 had 8.35% and 38.81% missing data, respectively. The maximum likelihood tree, estimated under two models, LG + C20 + F + Γ (C20) and LG + F + I + G4 (LG; the best-fitting model as determined by IQ-TREE), with 1000 standard bootstraps (BS) and 1000 ultrafast bootstraps (UFB). The tree topology shown is from the C20 analysis. Support values for < 50% BS and < 75% UFB are denoted by a dash (-), whereas an asterisk (*) marks a topology that does not exist in a particular analysis. Fully supported nodes are shown as black circles