Fig. 2
From: An epilepsy-causing mutation leads to co-translational misfolding of the Kv7.2 channel
The W344R mutation modestly reduced HA-Kv7.3 binding to CFP-Kv7.2. Co-IP of HA-Kv7.3 with Kv7.2-WT, I340E, or W344R mutants with N-terminus CFP tag. a Representative immunoblot of Co-IP in HEK293T cells co-transfected with 1 μg of pcDNA3-HA-Kv7.3 and 0.5 μg pcDNA3.1-CFP-Kv7.2 WT or I340E, or 1 μg pcDNA3.1-CFP-Kv7.2 W344R (see Additional file 1: Figure S2). The immunoblots show CFP-Kv7.2 monomers (~ 130 kD) and multimers, even though the immunoprecipitated fractions and lysates were treated with sodium dodecyl sulfate (SDS) sample buffer and the strong reducing agent TCEP. b Quantification of immunoblots (n = 5). GAPDH served as a loading control and all samples were normalized to WT with WT being 100%. Data represents the mean ± SEM (**p < 0.01, ***p < 0.005 vs CFP-Kv7.2-WT)