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Fig. 3 | BMC Biology

Fig. 3

From: An epilepsy-causing mutation leads to co-translational misfolding of the Kv7.2 channel

Fig. 3

The W344R mutant severely reduced surface expression of heteromeric HA-Kv7.3/Kv7.2 in the axons of cultured hippocampal neurons. a Representative images of surface HA-Kv7.3 as pseudo-color (upper panel), intracellular CFP-Kv7.2 WT or mutants (middle), and the AIS marker 14D4 (bottom) from neurons transfected with HA-Kv7.3 and CFP-Kv7.2 WT, I340E or W344R. Pseudo-color represents different levels of surface HA-Kv7.3 signal intensity as indicated by the calibration bar. Arrow indicates the locations of AIS. Scale bar, 25 μm (see Additional file 1: Figures S3-S4). b Background-subtracted fluorescent intensities of surface HA-Kv7.3 from transfected neurons were normalized to those of HA-Kv7.3/CFP-Kv7.2-WT. c The axon/dendrite ratio was computed for surface HA-Kv7.3/CFP-Kv7.2 fluorescent intensities. Sample numbers for b and c are WT (n = 19), I340E (n = 14), and W344R (n = 13). ***p < 0.005

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