Fig. 7

Multimerized binding sites for the three polydactyl zinc finger architectural proteins induces a local reduction in histone H3 occupancy. a Maps of transgene constructs used for the histone H3 ChIPs: E-w, bxd, Su, CTCF, Pita, Su-bxd, CTCF-bxd, Pita-bxd. The location of the sequences amplified by qPCR in X-ChIP experiments are indicated above the E-w construct map (1n, 2n, 3n, 4n, 5n, 6n). They are the same for all of the constructs tested. b X-ChIPs were performed with chromatin isolated from heads of adult flies homozygous for transgenes inserted at 96E. X-ChIPs were performed with histone H3-specific antibodies or with the IgG control. The ordinate shows the percentage of target sequences in the immunoprecipitated material relative to the input DNA. The abscissa indicates the transgene construct and amplified primer pair. The ras (coding part of Ras64B gene) was used as an internal control