Fig. 4

Profiling of small RNAs in L. casei BL23 using mDOT-seq. a A workflow for preparing the C, U and N sRNA libraries. b Group distribution of predicted sRNAs in L. casei BL23. sRNAs that could be assigned to more than one group were termed as “mixed” sRNAs. All validated sRNAs are listed in Table 1. c DNAplotter map of genomic positions of predicted sRNAs. Moving inwards from black circle denoting the genome of L. casei BL23, second and third green tracks represent the identified sRNAs on the forward and reverse strands respectively, fourth and fifth grey tracks — CDSs on forward and reverse strands accordingly, sixth orange track — prophage sequences and CRISPR region, seventh track denotes the GC content (%) and the inner most eighth track highlights the GC skew [(G − C)/(G + C)] of the genome with a window size of 10,000 bp and a step size of 200 bp (in both cases pink is for below the average and yellow green is for above the average). d Venn diagram of sRNAs identified in the C, U and N libraries. e Northern blot validation of sRNAs predicted in all three libraries. Asterisk marks sRNA where multiple bands are visible. 5S rRNA loading controls are provided at the bottom