Fig. 1

Cross-species transcriptional comparison of human and rhesus bulk placental tissue. Volcano plots showing gene expression fold differences between human (n=6) and rhesus (n=4) term placental tissue from DE#1, using data mapped to a the human genome and b the rhesus genome. Dashed lines denote DE significance (padj<0.05) and fold change (|L2FC|>2) thresholds; genes passing padj threshold (green), L2FC threshold (cyan), both (magenta), or none (gray). c Venn-diagram depicting intersection of DEGs identified using data mapped to human genome (stripes) and rhesus genome (spotted) to identify intermediate human-upregulated (light blue) and rhesus-upregulated (light red) genes sets. d Venn-diagram depicting the intersection of the results from the three DE analyses, to identify the final set of 447 human-upregulated genes (blue), and 505 rhesus-upregulated genes (red) (Additional file 3). e Top 25 most significant differentially expressed (ranked by mean padj) human-upregulated (blue) and rhesus-upregulated genes (red); box plots depict average Log₂ fold change of each gene from the three DE analyses. f Differential expression results of HPGs. (Left) Proportion of placental marker genes analyzed (purple) or excluded from (brown) DE analysis. Analyzed genes are further classified as either not differentially expressed (not DE) (gray), human-upregulated (blue), or rhesus-upregulated (red). (Right) ENSEMBL-classification of HPGs excluded from DE analysis; “no rhesus ortholog” (orange), “low confidence rhesus ortholog” (yellow), or “no one2one rhesus ortholog” (green). g Heatmap of differentially expressed HPGs; human-upregulated genes (L2FC>2) are shown in blue and rhesus-upregulated genes (L2FC<2) are shown in red