Fig. 4

iRP-D26 and iRP-D28A represent two highly pure rhesus immortalized trophoblast cell lines. a IHC staining of gestational day 50 rhesus placental tissue for mononuclear trophoblast (KRT7 and CDH1), stromal (VIM), and immune cell (PTPRC) markers (DAB, brown); hematoxylin nuclear counter stain (blue). b IF staining of immortalized cell lines for KRT7 (red), CDH1 (green), VIM (green), and PTPRC (red); DAPI nuclear counterstain (blue); results show that iRP-D26 and iRP-D28A cells express known mononuclear trophoblast markers, KRT7 and CDH1 (n=3). c Bar graphs of qRT-PCR expression results; bulk rhesus placental samples (purple, n=3), iRP cell lines (green, n=8). Statistical significance was determined using two-sided unpaired t test with alpha of 0.05 (*p<0.05, **p<0.01)