Fig. 5

Identification and mapping of GN cell-type subpopulations across cerebellar regions. a t-SNE visualization of spots identified as EGL, ML/PCL, and IGL in Fig. 4a. b Scores of genes specifically associated with four GN states in ST spots. c Spatial locations of anatomical regions associated with the development of GNs: EGL, ML/PCL, and IGL. Spots are colored according to the layers. d Model at P7 for the cellular architecture of GNs in different development phases: a. GNPs in EGL, b. migrating GNs in the inner of EGL and ML/PCL, and d. mature GNs in IGL. c. Purkinje cells are shown in ML/PCL. e Violin plot for scores of selected genes corresponding to four cell states in three layers’ location. Color represents four cell states. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001; ^****P < 0.0001. P values were determined using two-sided unpaired Wilcoxon test. f Correlation between four states of GNs and three layers’ location. g Signature gene expression of GNPs (Math1 and Mki67), GNs I (Mvd), and GNs II (Cntn1) in the ST H&E image