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Fig. 4 | BMC Biology

Fig. 4

From: Unexpected organellar locations of ESCRT machinery in Giardia intestinalis and complex evolutionary dynamics spanning the transition to parasitism in the lineage Fornicata

Fig. 4

Characterization of GiVPS36A-HA subcellular location. A Cell periphery and cytosol. (I) Ventral and (II) dorsal optical slices of transgenic Giardia trophozoite expressing GiVPS36A-HA. All images were obtained using Laser Scanning Confocal Microscopy. All scale bars: 5 μm. B PVs. (I) Immunofluorescence assay of transgenic Giardia trophozoites labelled for GiVPS36-HA (green) after incubation with Dextran-TxR (magenta). (II) Distribution of co-localization parameters for GiVPS36-HA and Dextran-TexasRed labeling from ≥ 15 analysed cells. Mean values for each parameter are indicated. (III) Signal overlap analysis and co-localization coefficients calculated for all slices of the sample either for the whole cell or ROI. Scale bars: composite 5 μm and ROI 1 μm. All images were obtained using Laser Scanning Confocal Microscopy

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BMC Biology

ISSN: 1741-7007

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