Fig. 6
Diffusion analysis of RibE-mV and the impact of RibH on its confined motion. A EAMSD versus time plot for the Rib FP enzyme RibE-mV (original locus) in the presence and absence of RibH, as well as, for RibE-mV (ectopic locus) with and without native RibE or RibH being present. Datasets from the original loci and the ectopic loci were treated separately and equalized in terms of trajectory abundance. All results are summarized in Tables 1 and 2. B Comparative JD analysis for different strains producing RibE-mV (original locus) either in the presence (left) or absence (right) of RibH and for strains expressing ribE-mV ectopically with (middle) and without native RibE (right) or RibH (right to the middle) being present. To assess the existence of different diffusive subpopulations, we used a three-component Rayleigh distribution model and evaluated all possible models using statistical F-tests for nested models. The obtained results are summarized in Table 3. C Model prediction versus observation plots for Rayleigh distribution modeling of JDs shown on top in B. D Bubble plot illustrating the differences in relative proportions of subpopulations for both strains with respect to their mean DCs that are derived from JD analysis in B. E Proposed model for encapsulation of RibE by RibH pentamers assuming either an equilibrium between free and encapsulated RibE (posttranslational mechanism), a cotranslational encapsulation mechanism or a mixture of both mechanisms. F Subcellular analysis of confined and free trajectories for RibE-mV either in the presence or absence of RibH, as well as for RibE-mV (ectopic locus) with and without native RibE or RibH being present. Displayed are the results using the radii given in Table S2 with three, six, or nine consecutive steps of confinement. The normalized probability is given below the panel. G Speed map representations of RibE-mV (original locus) in the presence and absence of RibH, as well as for RibE-mV (ectopic locus) with and without native RibE or RibH being present. Displayed are the spatial distributions of single step diffusion binned over areas of 0.1 μm2 for normalized cell representations