Table 1 Charge analysis of Rib enzymes and their respective FP rib enzymes used for SPT in this study
Fusion enzyme/protein | N aa (pI) | N + charged aa (R, K) | N – charged aa (D, E) | Net charge monomer | Oligomeric state (MM in kDa) | Net charge of oligomeric state(s) |
|---|---|---|---|---|---|---|
RibDG | 361 (6.09) | + 39 | − 44 | − 5 | Tetramer (156.6) | − 20 |
RibDG-mV | 611 (5.92) | + 66 | − 78 | − 12 | Tetramer (267.29) | − 48 |
RibAB | 398 (5.64) | + 43 | − 58 | − 15 | Dimer (87.92) | − 30 |
RibAB-mV | 648 (5.67) | + 70 | − 92 | − 22 | Dimer (143.28) | − 44 |
RibE | 215 (5.87) | + 22 | − 26 | − 4 | Trimer (69.99) | − 12 |
RibE-mV | 465 (5.78) | + 49 | − 60 | − 11 | Trimer (152.99) | − 33 |
RibH | 154 (5.35) | + 14 | − 17 | − 3 | 60-mer (968.4); 5-mer (80.7) | − 180* − 15 |
RibH-mV | 404 (5.61) | + 41 | − 51 | − 10 | 60-mer (2628.23); 5-mer (219.02) | − 600* − 50 |
mV (fluorescent protein) | 239 (5.71) | + 27 | − 34 | − 7 | − 7 |