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Table 1 Charge analysis of Rib enzymes and their respective FP rib enzymes used for SPT in this study

From: Transcription-dependent confined diffusion of enzymes within subcellular spaces of the bacterial cytoplasm

Fusion enzyme/protein N aa (pI) N + charged aa (R, K) N – charged aa (D, E) Net charge monomer Oligomeric state (MM in kDa) Net charge of oligomeric state(s)
RibDG 361 (6.09) + 39 − 44 − 5 Tetramer (156.6) − 20
RibDG-mV 611 (5.92) + 66 − 78 − 12 Tetramer (267.29) − 48
RibAB 398 (5.64) + 43 − 58 − 15 Dimer (87.92) − 30
RibAB-mV 648 (5.67) + 70 − 92 − 22 Dimer (143.28) − 44
RibE 215 (5.87) + 22 − 26 − 4 Trimer (69.99) − 12
RibE-mV 465 (5.78) + 49 − 60 − 11 Trimer (152.99) − 33
RibH 154 (5.35) + 14 − 17 − 3 60-mer (968.4);
5-mer (80.7)
− 180*
− 15
RibH-mV 404 (5.61) + 41 − 51 − 10 60-mer (2628.23);
5-mer (219.02)
− 600*
− 50
mV (fluorescent protein) 239 (5.71) + 27 − 34 − 7 Monomer [36, 37] (26.89) − 7