Fig. 4
From: Mutant resources for functional genomics in Dictyostelium discoideum using REMI-seq technology
Generation of a pooled REMI library for parallel phenotypic analyses. A Schematic of pooled library generation. REMI transformations were plated out at limiting dilution to ensure clonal growth and avoid competition between clones. After clonal growth, mutants were pooled to generate frozen stocks that are available from the Dictyostelium stock centre. B, C Independent replicas of the pooled library are highly similar. Eight independent replicas of the pooled library were grown up and sequenced. Although the total number of insertion sites (B) and insertion sites that are unique to each library (C) is different, this is can be wholly accounted for by sequencing depth