Fig. 8

RNAi-mediated depletion of DOM-A affects mitosis and cytokinesis in Drosophila S2 cells. DAPI staining is shown in blue, α-Tubulin in green. A Quantitative analysis of defects; mock (white histograms), DOM-A depleted cells (black histograms). B From left to right: normal metaphase (mock), multipolar spindle (RNAi), and chromosome misalignments (RNAi). C Left panel: normal telophase (mock); right panel: chromatin bridge (RNAi). D left panel: normal telophase (mock); right panel: long intercellular bridge (RNAi). E Left panel: mononucleated cell (mock); right panel: binucleated cell (RNAi). Scale bar = 5 μm. The quantitative analysis of defects scored in RNAi-treated and mock treated cells is based on about 300 metaphases, telophases, or interphases, scored in at least three independent experiments (Table 3). *P < 0.05; **P < 0.005; and ***P < 0.0005 compared with the mock group by Fisher’s exact test