Fig. 2.

Morphology of the nervous system in 42-day-old juveniles of L. ruber visualized with CLSM and antibody staining against tyrosinated tubulin (yellow, A–D), FMRF-amide (magenta, A–D), and serotonin (green, G–J) as well as Sytox green nuclear staining (cyan, E, F) and in situ hybridization with probe against choline acetyltransferase (red, E, F). Entire animal in dorso-ventral projection with a focus on dorsal (A, G) and ventral (B, H) structures; anterior part of the animal in dorso-ventral projection with a focus on dorsal (C, E) and ventral (D, F, J) structures; inset in E shows ChAT expression in the proboscis (yellow arrowheads); I lateral projection of the entire animal. Anterior is to the top on all panels. Scale bars 20 μm. adn, accessory dorsal nerve; an, anterior nerve; asc, anterior sensory cell; cc, ciliated canal; co, cerebral organ; dbc, dorsal brain commissure; dbl, dorsal brain lobe; dc, dorsal commissure; dln, dorsal lobe neuropile; dlp, dorsal lobe perikaryon; dnc, dorsal nerve cord; lnc, lateral nerve cord; pb, proboscis; pc, posterior commissure; phn, pharyngeal nerve; pn, proboscis nerve; ppc, postpharyngeal commissure; sdl, superior branch of the dorsal lobe; sns, stomatogastric nervous system; vbc, ventral brain commissure; vbl, ventral brain lobe; vln, ventral lobe neuropile; vlp, vetral lobe perikaryon. White arrowheads indicate transverse ventral commissures, double white arrowheads pharyngeal sensory cells, and asterisks the mouth opening.