Fig. 7

Functional analysis and verification of known TF motifs in ATAC-seq footprints. a Venn diagram analysis of TF binding motif instances of ATAC-seq footprints combined with RNA-seq data. Functional classification of the intersection genes of TF binding motif instances of ATAC-seq footprints combined with RNA-seq data through FungiFun. b In vivo functional verification of putative known transcription factor targeting sites was carried out by artificially synthesized minimal promoters. The red dividing line represents the location of the footprints to be verified. From top to bottom, there were 4 AmyR, 3 PrtT, and 3 CBC box (CCAAT) target sites validated by phenotype plates in vivo. c The repressors, CreA, were functionally verified by an artificial promoter containing AmyR instances. The active sites of 4 CreA were verified by phenotype plates, respectively. d qRT-PCR analysis of the goxC gene driven by CreA targeted instances. The experiment was performed in biological triplicate and gpdA served as the endogenous reference gene