Fig. 4

MAPK inhibition eliminates many cell types. A UMAP plot of all DMSO and U0126 treated STAMPs at the mid-gastrula stage. B Lineage diagram of all STAMP clusters identified in control embryos at the 64-cell, 112-cell, and mid-gastrula stages. Red lineage branches are missing after treatment with U0126, black lineage branches remain after U0126 treatment, blue lineage branches are transfated to a unique state. * = p < 0.05, X = p > 0.05, ‡ = untestable. C UMAP plot of endoderm and TLC STAMPs at the mid-gastrula stages depicting the incomplete transfating of B-line endoderm. D Expression of endoderm and muscle markers in the U0126 treated B-line endoderm, DMSO-treated B-line endoderm, and DMSO-treated muscle lineages