Fig. 8

Northern blot analysis of two Pgt miRNA-like candidates. A Two miRNA-like candidates, miRL-1 (22 nts with 5′ adenine) and miRL-2 (22 nts with 5′ uracil) are shown with sRNA read coverage and predicted miRNA-like hairpin structures. B Northern blot analysis confirmed their downregulation in infected tissue comparing to germinated spores. The same blot was stripped and re-hybridized with Pgt U6 or wheat miR168 probes for use as the RNA loading control. The wheat miR168 band also indicates the position of 21-nt sRNA. C The sRNA-seq data shows that both miRNA-like candidates are highly abundant in germinated spores compared to late infection