Fig. 2
From: Horizontal gene transfer-mediated bacterial strain variation affects host fitness in Drosophila
A The thiamine biosynthesis pathway in acetic acid bacteria. B Overview of thiamine biosynthesis genes in the analyzed bacteria. Note that the function of thiF that appears to be missing in the strains of the upper row can be replaced by the function of the homolog MoeB (Rodionov et al., 2002) that we found in all strains analyzed. Genes forming one operon are separated by a hyphen. Genes from different loci are separated by slashes. C Synteny of the flanking regions of thiamine biosynthesis genes in Gluconobacter and Acetobacter. thiOSG are missing on the G. morbifer branch (II) at this locus. Thiamine biosynthesis genes are in blue. The hypothetical protein is of unknown function. D Right: The complete pathway to synthesize Thiamine-P (green) forms an operon on the G. morbifer branch (branch II); left: the phylogeny depicts the inferred evolutionary scenario on branch II. E Phylogeny of thiE. G. oxydans DSM2343, G. oxydans DSM2003, G. sp. P1C6_b, and G. cerevisiae DSM27644 have two copies of thiE, thiE1 (blue) and thiE2 (green). The phylogeny of thiE1 (blue background) is congruent with the core genome phylogeny. ThiE2 (green background) forms a distinct clade that is more distant than thiE from Acetobacter, indicating HGT from a distant clade. Node labels represent posterior probabilities as assessed by MrBayes v 3.2.6 [62]