Fig. 2

Identification of multiple splice variants of Pirmy. A RT-PCR amplification of Pirmy showed multiple amplicons in both testis and brain. The RT-PCR products from testis were cloned and sequenced to identify the splice variants. NTC is the non-template control. Bottom panel shows Gapdh control for checking the integrity and quality of RNA. B Colour coded line diagram showing extensive alternative splicing of Pirmy. The 80 splice variants (DQ907162, FJ541103-FJ541181) depicted here localize to Y: 4341127-4381724 (GRC m39). Each exon is represented by the same colour in different isoforms. Sizes of the exons are to scale. Top two lines show the representation of all exons present at this locus according to their order in the genomic sequence as e1, e2, etc. Line 2 indicates the nucleotide positions of each exon in a scenario where all the exons are present. The exons have been arranged in linear order. C BLAST analysis against mouse genome localizes the splice variants of Pirmy to NT_166343.2